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pcag smfp v5  (Addgene inc)


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    Structured Review

    Addgene inc pcag smfp v5
    Pcag Smfp V5, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pcag+smfp+v5/pCAG_smFP+V5+(Plasmid+%2359758)/pm41904125-265-40-47
    Average 93 stars, based on 13 article reviews
    pcag smfp v5 - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Plasmid Preparation:

    Article Title: Regionally mapped astrocytic responses to cortical and white matter stroke show differential roles in astrocyte-induced vascular remodeling.
    Article Snippet: Article Regionally mapped astrocytic responses to cortical and white matter stroke show differential roles in astrocyte-induced vascular remodeling

    Article Title: Astrocyte morphogenesis requires self-recognition.
    Article Snippet: Self-recognition is a fundamental cellular process across evolution and forms the basis of neuronal self-avoidance.. Clustered protocadherin (cPcdh) proteins, which comprise a large family of isoform-specific homophilic recognition molecules, have a pivotal role in the neuronal self-avoidance that is required for mammalian brain development.. The probabilistic expression of different cPcdh isoforms confers unique identities on neurons and forms the basis for neuronal processes to discriminate between self and non-self.

    Article Title: Investigating morphological and cytoskeletal dynamics of hippocampal astrocyte across progressive tauopathy
    Article Snippet: .. WPRE primers: fwd, GGCTGTTGGGCACTGACAAT; rev, CCGAAGGGACGTAGCAGAAG. pUCmini-iCAPPHP.eB was a gift from Viviana Gradinaru (Addgene plasmid # 103005 ; http://n2t.net/addgene:103005 ; RRID:Addgene_103005). pCAG_smFP V5 was a gift from Loren Looger (Addgene plasmid # 59758 ; http://n2t.net/addgene:59758 ; RRID:Addgene_59758). .. AAV retroorbital injection Systemic delivery of AAV-GfaABC1D-lck-smV5-4x6T-WPRE (Addgene:196416)) was performed as follows.

    Article Title: Antibodies to PfGARP kill
    Article Snippet: .. To add spaghetti monster V5 46 for 3′ tagging the coding sequence from pCAG_smFP-V5 (a gift from Loren Looger, Addgene 260 plasmid #59758) was amplified with oligos oJDD3484/oJDD3485 and cloned into pRR67 with NcoI/PspOMI to generate pRR69. ..

    Article Title: Astrocyte morphogenesis requires self-recognition
    Article Snippet: .. To generate GfaABC 1 D .Lck-smV5 and Lck-smMyc plasmids, cytosolic smFP-V5 and smFP-Myc DNA sequences were amplified by PCR from the pCAG-smFP-V5 (Addgene plasmid # 59758) and pCAG-smFP-Myc plasmid (Addgene plasmid # 59757). ..

    Article Title: Astrocyte morphogenesis requires self-recognition
    Article Snippet: .. Plasmids and AAVs To generate GfaABC1D.Lck-smV5 and Lck-smMyc plasmids, cytosolic smFP-V5 and smFP-Myc DNA sequences were ampli ed by PCR from the pCAG-smFP-V5 (Addgene plasmid # 59758) and pCAG-smFPMyc plasmid (Addgene plasmid # 59757). ..

    Bioprocessing:

    Article Title: Regionally mapped astrocytic responses to cortical and white matter stroke show differential roles in astrocyte-induced vascular remodeling.
    Article Snippet: Article Regionally mapped astrocytic responses to cortical and white matter stroke show differential roles in astrocyte-induced vascular remodeling

    Amplification:

    Article Title: Astrocyte morphogenesis requires self-recognition.
    Article Snippet: Self-recognition is a fundamental cellular process across evolution and forms the basis of neuronal self-avoidance.. Clustered protocadherin (cPcdh) proteins, which comprise a large family of isoform-specific homophilic recognition molecules, have a pivotal role in the neuronal self-avoidance that is required for mammalian brain development.. The probabilistic expression of different cPcdh isoforms confers unique identities on neurons and forms the basis for neuronal processes to discriminate between self and non-self.

    Article Title: Antibodies to PfGARP kill
    Article Snippet: .. To add spaghetti monster V5 46 for 3′ tagging the coding sequence from pCAG_smFP-V5 (a gift from Loren Looger, Addgene 260 plasmid #59758) was amplified with oligos oJDD3484/oJDD3485 and cloned into pRR67 with NcoI/PspOMI to generate pRR69. ..

    Article Title: Astrocyte morphogenesis requires self-recognition
    Article Snippet: .. To generate GfaABC 1 D .Lck-smV5 and Lck-smMyc plasmids, cytosolic smFP-V5 and smFP-Myc DNA sequences were amplified by PCR from the pCAG-smFP-V5 (Addgene plasmid # 59758) and pCAG-smFP-Myc plasmid (Addgene plasmid # 59757). ..

    Polymerase Chain Reaction:

    Article Title: Astrocyte morphogenesis requires self-recognition.
    Article Snippet: Self-recognition is a fundamental cellular process across evolution and forms the basis of neuronal self-avoidance.. Clustered protocadherin (cPcdh) proteins, which comprise a large family of isoform-specific homophilic recognition molecules, have a pivotal role in the neuronal self-avoidance that is required for mammalian brain development.. The probabilistic expression of different cPcdh isoforms confers unique identities on neurons and forms the basis for neuronal processes to discriminate between self and non-self.

    Article Title: Astrocyte morphogenesis requires self-recognition
    Article Snippet: .. To generate GfaABC 1 D .Lck-smV5 and Lck-smMyc plasmids, cytosolic smFP-V5 and smFP-Myc DNA sequences were amplified by PCR from the pCAG-smFP-V5 (Addgene plasmid # 59758) and pCAG-smFP-Myc plasmid (Addgene plasmid # 59757). ..

    Article Title: Astrocyte morphogenesis requires self-recognition
    Article Snippet: .. Plasmids and AAVs To generate GfaABC1D.Lck-smV5 and Lck-smMyc plasmids, cytosolic smFP-V5 and smFP-Myc DNA sequences were ampli ed by PCR from the pCAG-smFP-V5 (Addgene plasmid # 59758) and pCAG-smFPMyc plasmid (Addgene plasmid # 59757). ..

    Cloning:

    Article Title: A bridge-like lipid transfer protein is critical for generation of invasive stages in malaria parasites.
    Article Snippet: Other plasmids used for transfections in this work, mCh-FRBminiTurbo, GRASP1-mCh and pLyn-FRB-mCh-nmd3-BSD (Addgene #85796) were generated by previous studies32,73. .. Used as templates or backbones for further cloning reactions, pSLI-N-GFP-2xFKBP-loxP (Addgene #85792), pSLI-C-GFP-Sandwich (also pSLI-sandwich, Addgene #85790), pSLI-C-mNG-Sandwich (same as addgene #85792 with mNG replacing GFP), pSkipFlox (Addgene #85797), mScRab6__NLS-FRB, Sf3A2__mCh-Kelch13, pLyn-FRB-mCh-nmd3-BSD (Addgene #85796), were previously generated in our laboratory32,33, pCAG_smFP-V5 was a gift from Loren Looger (Addgene #59758), and GAPM1-GFP and GAP45-mCh60 were gifts from T. Gilberger (CSSB, Hamburg, DE). ..

    Article Title: A bridge-like lipid transfer protein is critical for generation of invasive stages in malaria parasites
    Article Snippet: Other plasmids used for transfections in this work, mCh-FRB-miniTurbo, GRASP1-mCh and pLyn-FRB-mCh-nmd3-BSD (Addgene #85796) were generated by previous studies , . .. Used as templates or backbones for further cloning reactions, pSLI-N-GFP-2xFKBP-loxP (Addgene #85792), pSLI-C-GFP-Sandwich (also pSLI-sandwich, Addgene #85790), pSLI-C-mNG-Sandwich (same as addgene #85792 with mNG replacing GFP), pSkipFlox (Addgene #85797), mSc-Rab6__NLS-FRB, Sf3A2__mCh-Kelch13, pLyn-FRB-mCh-nmd3-BSD (Addgene #85796), were previously generated in our laboratory , , pCAG_smFP-V5 was a gift from Loren Looger (Addgene #59758), and GAPM1-GFP and GAP45-mCh were gifts from T. Gilberger (CSSB, Hamburg, DE). ..

    Generated:

    Article Title: A bridge-like lipid transfer protein is critical for generation of invasive stages in malaria parasites.
    Article Snippet: Other plasmids used for transfections in this work, mCh-FRBminiTurbo, GRASP1-mCh and pLyn-FRB-mCh-nmd3-BSD (Addgene #85796) were generated by previous studies32,73. .. Used as templates or backbones for further cloning reactions, pSLI-N-GFP-2xFKBP-loxP (Addgene #85792), pSLI-C-GFP-Sandwich (also pSLI-sandwich, Addgene #85790), pSLI-C-mNG-Sandwich (same as addgene #85792 with mNG replacing GFP), pSkipFlox (Addgene #85797), mScRab6__NLS-FRB, Sf3A2__mCh-Kelch13, pLyn-FRB-mCh-nmd3-BSD (Addgene #85796), were previously generated in our laboratory32,33, pCAG_smFP-V5 was a gift from Loren Looger (Addgene #59758), and GAPM1-GFP and GAP45-mCh60 were gifts from T. Gilberger (CSSB, Hamburg, DE). ..

    Article Title: A bridge-like lipid transfer protein is critical for generation of invasive stages in malaria parasites
    Article Snippet: Other plasmids used for transfections in this work, mCh-FRB-miniTurbo, GRASP1-mCh and pLyn-FRB-mCh-nmd3-BSD (Addgene #85796) were generated by previous studies , . .. Used as templates or backbones for further cloning reactions, pSLI-N-GFP-2xFKBP-loxP (Addgene #85792), pSLI-C-GFP-Sandwich (also pSLI-sandwich, Addgene #85790), pSLI-C-mNG-Sandwich (same as addgene #85792 with mNG replacing GFP), pSkipFlox (Addgene #85797), mSc-Rab6__NLS-FRB, Sf3A2__mCh-Kelch13, pLyn-FRB-mCh-nmd3-BSD (Addgene #85796), were previously generated in our laboratory , , pCAG_smFP-V5 was a gift from Loren Looger (Addgene #59758), and GAPM1-GFP and GAP45-mCh were gifts from T. Gilberger (CSSB, Hamburg, DE). ..

    Sequencing:

    Article Title: Antibodies to PfGARP kill
    Article Snippet: .. To add spaghetti monster V5 46 for 3′ tagging the coding sequence from pCAG_smFP-V5 (a gift from Loren Looger, Addgene 260 plasmid #59758) was amplified with oligos oJDD3484/oJDD3485 and cloned into pRR67 with NcoI/PspOMI to generate pRR69. ..

    Clone Assay:

    Article Title: Antibodies to PfGARP kill
    Article Snippet: .. To add spaghetti monster V5 46 for 3′ tagging the coding sequence from pCAG_smFP-V5 (a gift from Loren Looger, Addgene 260 plasmid #59758) was amplified with oligos oJDD3484/oJDD3485 and cloned into pRR67 with NcoI/PspOMI to generate pRR69. ..



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